AB22™ Bacillus subtilis: Heat‑stable probiotic performance
Most probiotics don’t survive the demands of real‑world manufacturing. Heat, pH shifts, pressure, and storage destroy delicate strains long before they ever reach the gut, making true efficacy difficult to achieve outside of refrigerated capsules.
AB22™, available exclusively from Amplified Ingredients, withstands those challenges and continues to perform under pressure, maintaining strength and functionality where others fail. This resilient, spore‑forming strain of Bacillus subtilis remains viable through high heat, mixing, and storage—even in demanding applications like gummies, baked goods, and ready‑to‑drink beverages. It delivers measurable support for gut health, immune balance, and overall resilience in active formulations and real‑world conditions.
For formulators, AB22™ represents more than a breakthrough; it’s a proven solution for incorporating probiotic function into shelf‑stable, heat‑processed, or otherwise hostile product environments.
What is AB22™?
AB22™ is a proprietary spore‑forming strain of Bacillus subtilis collaboratively discovered by Amplified Ingredients for exceptional stability and performance. As a dormant spore, it can withstand heat, acidity, pressure, and moisture — conditions that rapidly destroy conventional probiotics. This allows AB22™ to remain viable throughout manufacturing, transport, and shelf life, then fully activate once it reaches the digestive tract.
Upon germination, AB22™ produces beneficial enzymes and metabolites that help restore microbial balance, support digestion, and reinforce the gut–immune connection. Its remarkable heat tolerance and survivability make it uniquely suited for modern functional products such as RTDs, gummies, snacks, baked goods, and even coffee pods — categories that were once incompatible with probiotic activity.
Researched benefits of AB22™
Extensive in‑vitro and application testing demonstrate that AB22™ delivers measurable advantages for gut and metabolic health while maintaining viability through conditions that destroy most probiotics.
Heat and pH resilience
Retains more than 99% viability through processing temperatures up to 400°F and remains stable across a broad pH range. This enables true probiotic function in categories once considered off‑limits.
Gut microbiome balance
Selectively promotes growth of beneficial Lactobacillus and Bifidobacterium species while reducing opportunistic strains such as E. coli and Clostridium difficile. Supports a healthier, more stable microbiome that resists imbalance.
Digestive enzyme activity
Produces protease, lipase, amylase, and phytase to aid macronutrient digestion and release bound minerals. Enhances nutrient absorption and overall digestive comfort for end users.
Immune modulation
Supports balanced cytokine signaling and stimulates protective IgA production in the gut mucosa. Helps maintain immune readiness and resilience without overstimulation, ideal for daily wellness formulations.
Glyphosate defense
Protects intestinal cells and helps repair the gut lining when compromised by glyphosate exposure. Provides resilience against one of the most pervasive environmental disruptors linked to inflammation and microbiome disruption.
Why source AB22™ through Amplified Ingredients?
Amplified Ingredients is the exclusive home of AB22™ Bacillus subtilis. We didn’t source this strain; we developed it, validated it, and proved its potential through rigorous testing. Our team continues to refine its application performance, ensuring consistency across formats and manufacturing conditions.
Quality Control
Every lot of AB22™ is produced under controlled fermentation and quality standards to guarantee potency, purity, and reproducibility.
Partnership & Insight
Beyond supply, Amplified provides the scientific insight, application guidance, and formulation support needed to help partners integrate AB22™ effectively, from early‑stage development through commercial production. For formulators seeking reliable probiotic performance without the usual stability trade‑offs, Amplified offers not just an ingredient but a partnership grounded in science and real‑world proof.